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Lecture

D - Graphs.xlsx

28 Pages
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Department
Biology
Course Code
Biology 2290F/G
Professor
Robert Dean

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Description
Wavelength DCPIP Red Dye 340 0.093 0.142 360 0.075 0.125 380 0.048 0.121 400 0.039 0.12 420 0.038 0.13 440 0.034 0.168 0.7 460 0.039 0.24 480 0.059 0.37 500 0.085 0.51 0.6 520 0.134 0.59 540 0.19 0.508 560 0.25 0.35 580 0.3 0.141 600 0.311 0.039 0.5 0.4 Absorption 0.3 0.2 0.1 0 340 390 440 490 540 590 Wavelength (nm) DCPIP Red Dye 590 DCPIP ConcenAtrasibance at 600nm 0 0 1 0.2 2 0.38 3 0.562 4 0.76 0.8 0.7 0.6 0.5 0.4 0.3 0.2 Absorbance at 600 nm 0.1 0 0 0.5 1 1.5 2 2.5 3 3.5 4 -5) DCPIP Concentration (10 Absorbance Transmittance 0 1 0.045 0.9 0.097 0.8 0.155 0.7 0.222 0.6 0.301 0.5 0.398 0.4 0.523 0.3 0.699 0.2 1 0.1 Transmittance 1 0.9 0.8 0.7 0.6 0.5 0.4 A0.3rbance 0.2 0.1 0 0 0.2 0.4 0.6 0.8 1 Transmittance Absorbance Log (% Transmittance) 0 2 0.045 1.95 0.097 1.9 0.155 1.85 0.222 1.78 0.301 1.7 0.398 1.6 0.523 1.48 0.699 1.3 1 1 1 Log (% Transmittance) 0.9 2.5 0.8 0.7 2 0.6 0.5 1.5 0.4 Absorbance 0.3 Log (% Transmittance) 1 0.2 0.1 0.5 0 1 1.2 1.4 1.6 1.8 2 0 0 0.5 L1g (% Transmit1.5ce) t2 Time Tube #1 Tube #2 Tube #3 Tube #4 Tube #5 0 0 0 0 0 0 0.5 0.08 0.068 0.042 0.027 0.021 1 0.116 0.099 0.075 0.052 0.026 1.5 0.169 0.133 0.105 0.073 0.036 2 0.2 0.163 0.127 0.091 0.046 0.25 0.2 Tube #1 0.15 Tube #2 Tube #3 0.1 Tube #4 Tube #5 0.05 0 0 0.5 1 1.5 2 2.5 Rate of DCPIP Reductiora(ionlmfio)dium hydrosulphite 0 0.00 0 0 0.036 0.00 9.70464E-09 0.00001 0.052 0.00 1.40178E-08 0.00002 0.075 0.00 2.0218E-08 0.00003 0.099 0.00 2.66878E-08 0.00004 0.116 0.00 3.12705E-08 0.00005 deltaA600 Photon FluencecRarected delt6E00 5.03/1000 /20 0.403 302.90 0.1765 7.56861E-06 3.80701E-08 1.90351E-09 0.307 137.80 0.1285 5.51029E-06 2.77168E-08 1.38584E-09 0.307 76.61 0.1285 5.51029E-06 2.77168E-08 1.38584E-09 0.383 46.84 0.1665 7.13979E-06 3.59132E-
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