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Lecture 4

Lecture 4.pdf

4 Pages

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Biology 3444F/G
Nusha Keyghobadi

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Lecture 4 Chapter 7Can think of molecular markers as an internal natural tagging systemMonarch once tag goes on a butterfly it identifies an individual Birds can put on a band for an individual Polar bear can put on collar for individualMolecular tools instead of putting a tag on the outside of the animal lets use the information already contained in the cell to determine the individual eg Grizzly bear hair trap experimentWoods et al 1999 set up barbed wire with meat as attractant so that it would pull hair Hair follicles contain cells and DNA Aggressive grizzly bearplay dead Aggressive black bearmake lots of noise and be aggressive back Species id Grizzly bears have a 1315 bp deletion in the mitochondrial control region when compared to the black bear Gender id Used 2 genes ZFXZFY and SRY sex determining gene in animals will only be found in males When we amplify this gene we only expect to see it in the males The problem with only using SRY is the PCR may not work because of human error Also coamplify ZFXZFY so we can see a product whether male or female acts as a control for PCRThese reactions were always set up by female technicians to avoid contamination Individual id Microsatellites used 6 loci that worked in both species of bears Even with only 6 loci the alleles that they got were all unique Probability of Identity if I had one individual with a particular genotype what is the probability another individual in that population will have that genotype Want to ideally have a lower probability than the expected population sizeIf we use enough variable loci then the Probability of Identity can become so low that it is essentially impossible to get two randomly chosen individuals with the same genotypeThe bear example in the first lecture is an example of Genetic capturerecapture studies Can use these toEstimate population sizeEstimate movements of individualsEstimate group dynamics
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