Biology 3444F/G Lecture Notes - Lecture 6: Dna Barcoding, Maturase K, Rubisco
24 Jul 2014
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Community ecology: study of patterns of co- occurrence of different species and the interactions among them. Patterns of species richness. Structure of food webs. Ecosystem ecology: study of the flow of energy and matter through biotic and abiotic components of ecosystems. Dna barcoding vary between species. Usually use cytochrome oxidase i gene which is on the mtdna. For seed plants use matk and rbcl as standard dna barcode. Hypothesis: specialized herbivores and pathogens limit population growth of tree species that would otherwise become dominant. Found most beetle species were specialized, few generalists. Microarray: tool for simultaneously gathering information about many (hundreds or thousands) of different dna sequences. Genotyping studies (snp analysis) Identifying what species are in mixed samples (soil, water, etc. ) Label dna from individual of interest, when it hybridizes for g mutation see fluorescence at that probe. If for c mutation see fluorescence at other probe. Heterozygous, see fluorescence at both spots.
