Biology 3444F/G Lecture Notes - Lecture 7: Gene Expression, Cichlid, Reverse Genetics
24 Jul 2014
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Better alleles will increase fitness. Different alleles of that gene, fitness is the same (e. g. microsatellites). Set up array and stick on short sequences. Extract genomic dna from samples of interest. Cut dna into small fragments (digestion or shearing) Label dna w/ fluorescent labels. Can fit thousand and thousand of these probes on a small array. Analyze the fluorescence quickly via cameras connected to computers. Gene expression study differs from genotyping studies by where the sample we hybridize our microarray with comes from. In gene expression studies it starts with expressed mrna, which becomes the source for what we hybridize our microarray with. Known sequence (prior genomic data) Unknown sequence (anonymous): take random genomic fragments and use those to construct probes and then do microarray experiment. The following are still only showing correlation, haven"t been able to establish function. Confirm expression: should verify microarray expression using second approach (pcr) Confirm function: compare to genes of known function in model species.
