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Lecture

Chemical and Biochemical Engineering 2290A/B Lecture Notes - Carbonic Anhydrase, Enzyme Kinetics, Chemical Kinetics


Department
Chemical and Biochemical Engineering
Course Code
CBE 2290A/B
Professor
Mita Ray

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Excelet
The Kinetics of Enzyme Reactions
Objective: To explore the chemical kinetics of enzyme mediated biochemical reactions.
(This is consecutive reactions with equilibrium in the first step.)
DEFINE: S E ES P
(place your cursor over the green cells above)
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Note on screen size
Start by reviewing some simple chemical kinetics!
Solver Add-in and Analysis ToolPak must be loaded! See "needed Add-ins tab!!!
KM
Sinex 2008

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Review of Chemical Kinetics
To be able to understand enzyme kinetics, you must know the basics of chemical kinetics.
How does a catalyst work?
w/o cat enzyme
reactants
19 -10 -10 FALSE
activated complex
246.2 -10 -10
products
318.3 -10 -10
FALSE
without catalyst For more info on PE
with catalyst diagrams -
with enzyme
How does the concentration of a reactant influence the rate of a chemical reaction?
zero first second 0
0 -1 -1 -1
0.1 -1 -1 -1
0.2 -1 -1 -1
0.3 -1 -1 -1
0.4 -1 -1 -1
0.5 -1 -1 -1
For more on rates, see the
Chemical Kinetics Simulation -
For more on consecutive reactions -
Sinex 2008
10
20
30
40
50
reaction coordinate
PE
concentration, M
Rate, M/sec

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Enzyme Kinetics
0 ### Michaelis-Menten equation Select enzyme
2
1 1.20E-02 Does this look like any typical reaction?
(S) (S)
0### 0 1
### 2.94E-01 0.01 1
1.00E-02 4.55E-01 locating Km 0.01 1initial
1.50E-02 5.56E-01 0.000 0.5 0.02 1rate of
### 6.25E-01 ### 0.5 0.02 1reaction
### 6.76E-01 ### 0 0.03 1This is the
### 7.14E-01 0.03 1rate at the
### 7.69E-01 0.04 1very start
### 8.06E-01 x-axis 0.05 1of the
### 8.54E-01 scale factor 0.07 1reaction;
1.00E-01 8.93E-01 10 0.10 1hence the
1.50E-01 9.26E-01 0.15 1initial rate
2.00E-01 9.43E-01 0.20 1
The (S) axis will rescale
based on the enzyme
selected. substrate
TRUE TRUE carbon dioxide
How does the order of the reaction behave?
answer
x-axis
(E)o =
kcat =
vmax =
(- - - -)
KM =
vo
vmax
vo =
at (S)o.
Sinex 2008
0.0E+00
2.0E-01
4.0E-01
6.0E-01
8.0E-01
1.0E+00
1.2E+00
(S)
vo
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