Objective: to explore the chemical kinetics of enzyme mediated biochemical reactions. (this is consecutive reactions with equilibrium in the first step. ) Km (place your cursor over the green cells above) There are a total of thirteen (13) tabs to explore!! Solver add-in and analysis toolpak must be loaded! To be able to understand enzyme kinetics, you must know the basics of chemical kinetics. How does a catalyst work? w/o cat enzyme. Pe reaction coordinate without catalyst with catalyst with enzyme. How does the concentration of a reactant influence the rate of a chemical reaction? zero first second. The (s) axis will rescale based on the enzyme selected. (e)o = This is the rate at the very start of the reaction; hence the initial rate at (s)o. Enzyme kinetics data follow a hyperbolic path, where they asymptotically approach the vmax as the enzyme becomes saturated. How does the order of the reaction behave? answer.