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Lecture

Chemical and Biochemical Engineering 2290A/B Lecture Notes - Phosphorylase, Buffer Solution, Molar Attenuation Coefficient


Department
Chemical and Biochemical Engineering
Course Code
CBE 2290A/B
Professor
Mita Ray

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Week$4$:$Study$the$effect$of$substrate$concentration$on$enzyme$activity$
$
Protocol:$You$are$studying$the$effect$of$substrate$concentration$on$rate$of$enzyme$
activity.$
Assay+Mixutre:$
Kinetic+Assay:++
3.9ml$Buffer$A$
1ml$substrate$
0.1ml$enzyme$
Read$A410$nm$every$30$seconds$for$4$minutes$
We’ve$provided$you$with$5$different$concentrations$of$substrate:$$
2.7mM,$1.35mM,$0.675mM$,$0.3375mM,$0.16875mM$
Time (min)
[S]=
2.70mM
[S]
=1.350mM
[S]
=0.675mM
[S]
=0.3375mM
[S]
=0.16875m
M
0
1
1.5
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