School
Western UniversityDepartment
Chemical and Biochemical EngineeringCourse Code
CBE 2290A/BProfessor
Mita RayThis preview shows half of the first page. to view the full 2 pages of the document.
Week$2$:$Study$the$effect$of$pH$on$enzyme$activity$
$
Protocol:$you$will$be$monitoring$the$time$of$enzymatic$digestion$under$three$
different$pH$treatment.$They$will$all$be$done$at$room$temperature.$
Assay+Mixutre:$
Kinetic+Assay:++
3.9ml$Buffer$A$
1ml$substrate$
0.1ml$enzyme$
Read$A410$nm$every$30$seconds$for$4$minutes$
$
Time$(min)$
Buffer$A$
Buffer$B$
Buffer$C$
$$
$$
$$
$$
$$
$$
0$
$$
$$
$$
$$
$$
$$
$$
$$
$$
1$
$$
$$
$$
$$
$$
$$
$$
$$
$$
1.5$
$$
$$
$$
$$
$$
$$
$$
$$
$$
2$
$$
$$
$$
$$
$$
$$
2.5$
$$
$$
$$
You're Reading a Preview
Unlock to view full version