Chemical and Biochemical Engineering 2290A/B Lecture Notes - Reaction Rate
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Published on 14 Nov 2011
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Week$2$:$Study$the$effect$of$pH$on$enzyme$activity$
$
Protocol:$you$will$be$monitoring$the$time$of$enzymatic$digestion$under$three$
different$pH$treatment.$They$will$all$be$done$at$room$temperature.$
Assay+Mixutre:$
Kinetic+Assay:++
3.9ml$Buffer$A$
1ml$substrate$
0.1ml$enzyme$
Read$A410$nm$every$30$seconds$for$4$minutes$
$
Time$(min)$
Buffer$A$
Buffer$B$
Buffer$C$
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0$
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1$
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1.5$
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2$
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2.5$
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