Chemistry 2223B Lecture Notes - Amphoterism, Zwitterion, Amine
18 May 2014
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Draw the predominant form(s) of the amino acid produced after the addition of 0. 0, 0. 5, 1. 0, 1. 5, 2. 0, 2. 5, and 3. 0 equivalents of hcl. In order to work out the structure of a protein, a necessary first step is to analyze for the amounts of each amino acid contained in it. The graphic below represents the recording from an amino acid analyzer, run at ph 5. 5 using the column type discussed in the class notes (negatively charged and hydrophobic). Briefly describe how the elution order would change if the column were positively charged and hydrophobic, instead of being negatively charged and hydrophobic. Assume that the experimental conditions (ph and solvent) are the same. Ninhydrin reacts with most -amino acids to produce a purple compound. All but one of the natural amino acids react with ninhydrin (i) to give a purple pigment (ii). Leu: which of the following can react with sanger"s reagent (dinitrofluorobenzene)? a)
