Chemistry 2223B Lecture Notes - Lecture 6: Dna Sequencing, Photographic Film, Cell Nucleus
15 Mar 2015
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Document Summary
N atoms 1 & 9 in pyrimidines & purines respectively used to make n-glycosidic linakges. Bases attached to sugars as -n-glycosides (n1 pyrimidines, n9 purines) Phosphates can be attached to one or more of oh groups (2", Prime refers to the numbering of sugar pka1 of each phosphate group is < 5 pka2 of each phosphate (if there is a second proton is about 7) Dna has 2 anti-parallel strands in right-handed helix. A-t & g-c base pairing by h-bonds. Molecules can recognize h-bonds donors & acceptors in grooves & selectively bind binding methods include non-selective interactions (ion-pairs) with negatively charged phosphate ester backbone & intercalation where a molecule inserts itself between base pairs. Reactivity of dna & rna acid & bases. Both dna & rna can be hydrolyzed under proper acidic conditions, as they possess acid-labile functional groups (phosphate esters & n-glycosidic linkages)
