Medical Biophysics 3501F Lecture Notes - Lecture 7: Venule, Motion Blur, Skeletal Muscle
5 Oct 2015
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Ivm inverted microscope: instead of objective is above the sample, it is below the sample. Practical advantages: cell culture monolayer of cells growing in a petri dish, can see the sides they are adhered to. Functional capillary density (how many cells are flowing in the vessel per unit time) Rbc lineal density tube hematocrit (linear density/volume ) Get a single vessel in our view consider the center line pixels that"s our position longitudinally along the vessel. The video sequence: sequence of video frames/individual images. Have a matrix of numbers + pixel values. We plot the pixel intensity along the centre line position for every frame = get a space time image. Space on the y axis, time on the x axis changed this to a 2d image that includes it over time. The black lines = the transit of an individual/group of cells from the entrance to the exit of this vessel.
