Medical Sciences 4900F/G Lecture Notes - Lecture 5: Lysis Buffer, Western Blot, Immunoprecipitation
22 Apr 2019
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Document Summary
Macromolecules made up of chains of amino acids. Perform specialized functions within the body (e. g. transporting molecules, catalyzing reactions. Proteins (e. g. enzymes) are 3d they consist of linear chains that undergo folding to arrive at 3d structures. Proteins are essential component of organisms and they participate in most cellular processes. There are many goals in protein research: Isolating and purifying protein: detecting protein expression, elucidating molecular structure, determining cellular localization, studying proteomics, analyzing structure and function (bioinformatics) There are multiple techniques that can be used to detect proteins in a biological sample. These include but are not limited to: Immunoprecipitation: western blot, bca assay, spectrophotometry, enzyme assays. In this course we will be performing western blot to detect certain proteins within our sample. Before we perform a western blot, we must isolate protein from our sample. Proteins can be extracted or isolated from tissues or cells. Overview of the protocol for protein isolation for cells.
