Pharmacology 3620 Lecture Notes - Lecture 43: Minimum Inhibitory Concentration, Rifampicin, Staphylococcus Epidermidis
14 Apr 2017
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Document Summary
Therapy against microorganisms (mos) takes advantage of biochemical differences between mammalian (eukaryotic) and bacterial cells to reduce toxicity to the patient. Criteria for selection of antibiotics: identity of the mo, susceptibility of mo to a given drug, site of infection, patient factors, safety of drug, cost of drug. Analysis of fluid/tissue samples from the patient blood, csf, synovial, peritoneal fluids, urine. Common laboratory techniques used include: direct microscopic visualization, cultivation and identification of mo, detection of microbial antigens, detection of microbial rna or dna, detection of host immune response (i. e. presence of antibodies in patient sample) Some mos have predictable patterns many don"t. Therefore, the minimum inhibitory concentration (mic) & minimum bactericidal concentration (mbc) needs to be experimentally determined. Steps to determine mic & mbc once the pathogen has been identified: Inoculate tubes with increasing antibiotic concentrations with mo. Incubate & determine lowest concentration of antibiotic that inhibits bacterial growth mic.
