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Canada (511,185)
Physiology (1,062)
Tom Stavraky (262)
Lecture

bone stuff.doc

3 Pages
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Department
Physiology
Course Code
Physiology 3120
Professor
Tom Stavraky

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Description
- Melatonin dose-dependently augmented proteins that are incorporated into the bone matrix, like procollagen type I c-peptide. Osteoprotegerin, an osteoblastic protein that inhibits the differentiation of osteoclasts is also augmented by melatonin in vitro - Another possible target cell for melatonin is the osteoclast, which degrades bone partly by generating free radicals. Melatonin through its free radical scavenger and antioxidant properties may impair osteoclast activity and bone resorption - http://apps.isiknowledge.com/CitedFullRecord.do? product=WOS&db_id=WOS&[email protected]&search_mode=CitedFull Record&isickref=87947327 http://www.jbc.org/cgi/reprint/279/6/4642 Role of Cholesterol in the Regulation of Growth Plate Chondrogenesis and Longitudinal Bone Growth Hormonal Regulation of Bone Growth The majority of the long bones of the body (including the tibia) form through a process called endochondral ossification. A cartilage template is first formed in development and is then replaced by bone. For continued growth of these bones, a cartilaginous growth plate persists until the end of puberty in humans, and is responsible for longitudinal growth. A multitude of hormones and other proteins regulate these processes and can increase or decrease growth rate. Glucocorticoids, cholesterol and melatonin all have varying effects in the body, including the regulation of bone growth. To study the effect of these compounds, students will learn to dissect embryonic tibia from mice, grow them in tissue culture and administer the compounds. Longitudinal growth will then be assessed and the end of the culture period. Objectives: In the lab you will… 1. Learn and be able to describe how the growth plate controls longitudinal growth of bones. 2. Research the known molecular effects of various compounds on chondrocytes (the cell type of cartilage) and then predict the effect on longitudinal growth. 3. Learn how to microdissect tibia from embryonic mice. 4. Learn and practice sterile technique for culturing tissues. 5. Examine the effects of compounds on longitudinal growth of long bones. Abstract Purpose/Hypothesis – The purpose of this experiment was to study the effects of dexamethasone, melatonin, and cholesterol on the regulation of embryonic tibia growth. We hypothesized that dexamethasone would inhibit bone growth while melatonin and cholesterol would promote bone growth. Method– After arresting CD1 embryonic mice at day 15.5 of gestation, tibia were microdissected and isolated on individual growth plates. Within each independent trial, tibia were subdivided into 5 treatment groups: DMSO cont
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