Psychology 3226A/B Lecture Notes - Lecture 2: Immunocytochemistry, Autoradiograph, Cholecystokinin

• School: Western University
• Department: Psychology
• Course: Psychology 3226A/B
• Professor: Genevieve Farrell

Document Summary

Bioassays inject hormone sample into living system and then measure a change (physiological change, typically) use a living system to measure hormones in a sample takes advantage of physiological response crop sac of pigeon can be used to measure prolactin (prl) of rat: crop sac prolactin assay (inject pigeons with varying doses of prolactin) creates dose response curve issues: if there is a problem with control sample, this will impact the dose response curve; must do it in live animal (probably have to euthanize afterwards, pigeons make crop milk to feed their young, the cells oanrogf the sac look like figure b) when not producing milk, prolactin is associated with parental behavior, increased prolactin makes the epithelium much thicker rabbit test (how we used to tell if women were pregnant urine from women into rabbit, inject urine into rabbit, hcg induce corpora lutea formation in ovaries.