BI346 Lecture Notes - Lecture 12: Transcription Bubble, Ribonucleoside, Dna Replication

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2 strands important for which one is copied. If you used the template strand = reverse complement and it would not work to use this one! To recognize the coding strand vs. template strand(rna polymerase will bind to based on the sequence of the template, so that the new strand will be the same as the coding strand) There"s no nucleolus in pro so translation can occur right away. In euk due to internal membrane, transcription and translation is separated temporally and spatially. Upstream (before gene) is a negative number, ex -10. Rna reading opposite to the way its transcribing (5" to 3") With rna polymerase there is no clamp or clamp loader. Promotor clearance is needed for full length transcript. Terminator sequence tells rna polymerase to stop and it will dissocated and it will be finished. More complex regulation in transcription than dna replication. Only required rna at the right time (to not waste)

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