BI346 Lecture Notes - Lecture 9: Dna Replication, Helicase, Primase
Document Summary
Look for start (atg) and stop codon (taa, tag, tga) Need a start in combination with a tata box. What it most likely encodes ^^ will be tested. Making more dna, multiple copies of genome in preparation of separation. Initiation: origin, dna is open and unwound, dna replication forks (either side of the bubble) In the bubble they are regionally signal stranded. Dna polymerases are directional (only 5" to 3" direction) Leading strand moves according to daughter (5 to 3) Lagging strand moves 5 to 3 as well, but they are moving away from the replication forks and this leads to okaski forks. Continues until termination sequence slide 12 formation of replication bubble. Two proteins (yellow) are helicases and they help the fork move bi directionally, un-winiding. Primase sets and rna primer helps it be extended. They are processive, it remains associated with dna and synthesizes long tracks of dna.
