BI226 Lecture Notes - Gametophyte, Spermatozoon, Spermatogonium

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9 Sep 2014

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The duration of the s phase can be determined with a pulse-chase experiment. Any cell that are in the s phase will have a radioactive substance in the nuclei that will appear in the g2 phase. Once it gets to the g2 phase, the timer starts. When you no longer see them labels in g2, the time stops and that"s the length of time in mitosis. 1/10 of cells in mitosis and mitosis takes an hour therefore the length of the cell cycle is 10 hours. Mitosis occurs in both haploid and diploid cells. Outcome two cells, each with the same number and type of chromosomes as the original parent cell. If you start with a haploid cell, you get two haploid cells; start with diploid and you will have 2 diploid cells in the end. The process is divided into four cytologically distinguishable phases:

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Question 3 (5 points)

Match the terms to the definitions provided.

Question 3 options:

	telophase II
	meiosis II
	telophase I
	anaphase I
	anaphase II

1.	Division, beginning without DNA replication, that resembles and produces four haploid cell.s
2.	Phase of meiosis when chiasmata are broken as spindle fibers shorten and homologous pairs are pulled apart.
3.	Phase of meiosis when sister chromatids are pulled to opposite poles of the cells.
4.	Phase of meiosis in which homologues cluster at the poles of the cells and the nuclear envelope reforms.
5.	Phase of meiosis in which four different haploid cells are present.

Question 4 (7 points)

Match the terms to the definitions provided.

Question 4 options:

	G0
	telophase
	G2
	mitosis
	cytokinesis
	cell cycle
	S

1.	The phase in which the cell synthesizes a replica of its genome
2.	The overall process of genome duplication
3.	The phase in the cell cycle during which mitochondria and other organelles replicate.
4.	The phase in mitosis when chromosomes are clustered at opposite poles and the spindle apparatus disappears
5.	A resting state that cells often enter before resuming cell division.
6.	The phase in the cell cycle when the cytoplasm divides creating two daughter cells.
7.	The phase in the cell cycle in which the spindle apparatus assembles and sister chromatids move apart.

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Question 5 (6 points)

Put the events of a MAP kinase cascade in the correct order.

Question 5 options:

	MAPKKK phosphorylates and activates another kinase
	MAPKK phosphorylates and activates MAPK
	Binding of MAPKKK to Ras
	External signal binds to membrane receptor and activates receptor kinase.
	MAPK phosphorylates an inactive response protein and activates it.
	Phosphorylation of receptor kinase and interaction with RAS

Question 1 (2 points)

In which phase of cell division is marked by the dissolution of the nuclear membrane, the condensation of chromosomes, and the formation of the mitotic spindle?

Question 1 options:

	prophase
	metaphase
	telophase
	anaphase

Question 2 (2 points)

The physical separation of cells during cell division is called:

Question 2 options:

	Cytokinesis
	Telophase
	Interphase
	Chromosome
	Cell plate

Question 3 (2 points)

Meiosis is necessary for sexual reproduction; the gametes formed during this process before fertilization are _______________.

Question 3 options:

	Haploid
	Diploid
	Zygote
	Homologous
	Tetrads

Question 4 (2 points)

Mitosis is the process by which a ___________ cell separates the chromosomes in its nucleus into two identical sets of nuclei.

Question 4 options:

	eukaryotic
	prokaryotic
	bacterial
	all of these are correct

Question 5 (2 points)

Which of the following statements about crossing over is INCORRECT?

Question 5 options:

	It occurs between homologous chromosomes
	It allows for two parents to produce many different types of offspring
	It contributes to genetic diversity in a population
	It occurs during prophase I of mitosis

1)What experiment was performed that allowed for scientists to finally agree that DNA is the genetic material within living organisms?

a.Understand the materials and scientific techniques used to come to this result.

2)What group of experiments led to the discovery of the mechanism used in DNA replication?

a.Understand the materials and scientific techniques used to come to this result.

i.What is a cesium chloride gradient?

ii.Where do 14N and 15N respectively fall along this gradient?

b.The results revealed that DNA replicates using which of the three proposed models?

i. Describe the mechanism of each model.

3)What is the role of the following proteins in DNA replication?

a.Helicase

b.Single strand binding proteins

c.Topoisomerase

d.DNA polymerase III

e.DNA polymerase I

f.Primase

g.Ligase

4)What extra steps are taken to be sure the lagging strand does not shorten each time DNA is replicated?

a.What is the role of telomerase in these âstepsâ?

5)In DNA repair, which enzyme has âproof-readingâ capabilities?

1)Which type of cells within our bodies use mitosis to reproduce?

2)Does mitosis create a cell that is different or the same as the parent cell undergoing division?

3)Describe the DNA found in sister chromatids.

4)Describe the DNA found in homologous chromosomes.

5)What occurs during the following stages of the cell cycle?

a.G₁ Phase

b.S Phase

c.G₂ Phase

d.M Phase

What are the phases of mitosis? What events occur in each phase?

1)Does mitosis separate homologous chromosomes, sister chromatids, or both?

2)What is cytokinesis?

a.Which fibers of the cytoskeleton contract to pinch parent and daughter cells apart from one another in animals?

b.How do microtubules move chromosomes to the two poles of a dividing cell?

3)How are centrioles involved in this process?

a.How do plants form two complete cells during cytokinesis?

4)How do bacterial cells reproduce?

5)The cell cycle is controlled at âcheckpointsâ. What occurs at each of the following checkpoints?

a.G₁ Checkpoint

b.G₂ Checkpoint

c.M Checkpoint

6)How do cyclin and cdk act to control the G2 checkpoint?

7)If presented with the schematic of the M checkpoint, you should be able to explain perturbations to the system as well as if the cell will divide or stall at metaphase.

8)Cancerous cells form when the cell cycle checkpoints do not function correctly and continues to divide even though it is damaged in some way. How does the protein p53 function to keep these cells from dividing?

9)Normal cells follow certain rules of growth that cancerous cells do not follow. What are these rules (provide a short definition for each as well)?

10)What is the difference between a benign tumor and a malignant tumor?

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BI226 Lecture Notes - Gametophyte, Spermatozoon, Spermatogonium

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