CH250 Lecture Notes - Lecture 10: Antigen, Polyclonal Antibodies, Thymus
Document Summary
Ligand -a molecule bound reversibly by a protein. Binding site - a ligand binds at a site on the protein. Is complementary to the ligand in size, shape, charge, and hydrophobic or hydrophilic character. Different ligands may have separate binding sites for several. Induced fit - the structural adaptation that occurs between protein and ligand is called. Enzyme changes shape to fit better with the ligand when it binds to. In a multisubunit protein, a conformational change in one subunit often affects the conformation of other subunits. Oxygen is poorly soluble in aqueous solutions. Diffusion of oxygen through tissues is also ineffective over distances greater than a few millimeters. However, none of the amino acid side chains in proteins is suited for the reversible binding of oxygen molecules. Heme is buried deep within protein to prevent irreversible oxidation of ferrous ion (simultaneous reaction of one o2 with two heme molecules will result in oxidation)
