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BCHM 2021 Lecture Notes - Lecture 15: Cytosol, Myofibril, Neuromuscular Junction

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ivoryeel382
21 Oct 2012
School
York University
Department
Biochemistry
Course
BCHM 2021
Professor
Julie Clark

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Document Summary

On microtubules and they walk to the plus end most few. Kinesin go to the minus end there is a binding site on the organelle or microtubules. Tail has the binding site if it attaches to organelles . Question: there are no known motor proteins that move on intermediate filaments. Answer: intermediate filaments have no polarity, but actin filaments and microtubules do. Motor proteins wouldn"t have a sense of direction on intermediate filaments. **video 16. 8-myosin*: filament binding, large conformational change in, release from filament, relaxation of conformation, filament rebinding. Lever arm moves: no atp, tight binding to actin, atp binds, releases actin, conformational change. 4)binds to actin pi release power stroke: adp leaves tight binding to. Answer: atp is rapidly hydrolysed when cells are deprived of oxygen. In the absence of atp, myosin binds tightly to actin. Two heads work together: front head loses adp and binds to the atp tight binding to tubulin, conformational change rear head forward.

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Related Questions

To examine the directionality of a kinesin motor that you havepurified, you attach the motor to a microscope slide. As yourmotility substrate, you add red fluorescent taxol-stabilizedmicrotubules that have been used to nucleate assembly of greenfluorescent tubulins. You add these red/green microtubules to theslide in the presence of ATP. What do you observe?

1. The microtubules move with red fluorescence at the front end ofthe microtubules.
2. The microtubules move with green fluorescence at the front endof the microtubules.
3. The microtubules move, and the green and red together produceyellow.
4. No motility since microtubules require GTP.
5. None of the above.

Explain how the microtubule binding activity of dynein, dynactin, and kinesin II is regulated by specific kinases and phosphatases during organelle transport.

Themicrotubule and actin microfilament cytoskeletons use molecularmotors for transporting cargo (vesicles, organelles) through thecytosol. Speculate on why two families of motors (kinesin anddynein) are associated with microtubule-based transport, while onlyone family of motors (myosin) is associated with actinmicrofilament function has only one. Drawings may be used to helpyour explanation.