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Lecture 3

BIOL 1000 Lecture Notes - Lecture 3: Methylene Blue, Pressure Measurement, PipetteExam


Department
Biology
Course Code
BIOL 1000
Professor
Nicole Nivillac
Lecture
3

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Shiyanuka Raveendrakumar
215615990
Partner: Iman Aden
Other Group: Rebecca, Fatima (Table 2)
Table 1. The cumulative oxygen production (mL O2) of the aquatic plant, cabomba caroliniana under
white light, is used to determine the photosynthetic rate (mL O2/g/hour). Experimental tubes 1 and 2
contained approximately the same amount of aquatic plant whereas the control tube underwent the same
processes without the aquatic plant. All tubes were experimented twice with pond water, and white light
under room temperature (23ºC). A manometric apparatus is a closed system that does not allow any gases
to enter or escape, and was used to record the oxygen production. This was measured through the change
in pressure shown by the displacement of methylene blue in a pipette. The photosynthetic rate is the
cumulative oxygen production rate which is determined by the slope (mL O2/hour) divided by the weight
(g). The cumulative oxygen production was recorded every 0.03 hours for a total of 0.27 hour. The
aquatic plant under white light showed a positive photosynthetic rate throughout both of the trials; this is
proven by the oxygen production which was increasing in increments every 0.03 hours.
Photosynthetic Rate of Cabomba Caroliniana in White Light
Time (Hours)
Slope
(mL
O2/ho
ur)
Weight
(g)
Photosynte-
tic Rate
(mL
O2/g/hour)
0.00
0.0
3
0.1
0
0.1
3
0.1
7
0.2
0
0.2
3
0.27
Trial
1
Experimental
Tube 1
0.00
0.0
2
0.0
6
0.0
8
0.1
1
0.1
3
0.1
5
0.17
0.0217
4.03
0.0054
Experimental
Tube 2
0.00
0.0
1
0.0
4
0.0
5
0.0
7
0.0
9
0.1
0
0.11
0.0147
4.00
0.0037
Control Tube
0.00
0.0
0
0.0
0
0.0
0
0.0
0
0.0
0
0.0
0
0.00
0
0.00
0
Trial
2
Experimental
Tube 1
0.00
0.0
2
0.0
6
0.0
9
0.1
3
0.1
4
0.1
6
0.18
0.0232
4.03
0.0058
Experimental
Tube 2
0.00
0.0
2
0.0
4
0.0
6
0.0
8
0.0
9
0.1
1
0.12
0.0152
4.00
0.0038
Control Tube
0.00
0.0
0
0.0
0
0.0
0
0.0
0
0.0
0
0.0
0
0.01
0.0007
0.00
0.0007
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Figure 1. The line of best fit (mL O2/hour) is shown through the plotted cumulative oxygen production
(mL O2) points over time (hours) of the aquatic plant, cabomba caroliniana, in white light. All tubes were
exposed to white light at room temperature (23°C) and contained pond water. All tubes with the exception
of the control tubes contained the plants, which is why the control tube had almost no oxygen production.
All tubes were in a manometric apparatus, which is a closed system that prevented any gas transfer into or
out of the system. This experiment was conducted twice. The oxygen production was recorded by
observing the blue methylene in the pipette as it moved away from the stopper while oxygen was
produced by photosynthesis. The slope represents the rate at which the oxygen was produced. The data
was collected over a duration of 0.27 hours in intervals of 0.03 hours. The trend shows a positive
correlation for the experimental tubes, thus the aquatic plant has a positive photosynthetic rate
(mLO2/g/hour) in white light.
-0.06
0.00
0.06
0.13
0.19
0.25
0.00 0.03 0.07 0.10 0.13 0.17 0.20 0.23 0.27
Cumulative Oxygen Production (mL O2)
Time (hours)
Experimental Tube 1 (Trial 1) Experimental Tube 2 (Trial 1)
Control (Trial 1) Experimental Tube 1 (Trial 2)
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