BIOL 2021 Lecture Notes - Lecture 9: Mannose, Phagocytosis, Surface 3

• School: York University
• Department: Biology
• Course: BIOL 2021
• Professor: Patricia Lakin- Thomas

Document Summary

Regulation of vesicle formation: lipid markers -> phosphoinolitides (most common) -> different proteins bind different pis, coat-recruitment gtpases. Fig 13-14a formation of copii coated vesicle: cop2 protein shown exiting the er, protein binding to pis, active when gtp bound and inactive when gdp bound (molecular switches) Activated via gef and inactivated via gap: examples. Arf -> helps make cop1 coat + clathrin at golgi. Sarl -> cop 2 at er exit site. Acivated via sar1-gef membrane -> picks up gtp which causes a conformational change -> binds to membrane as a sar1-gtp complex. Fig 13-14b formation of copii coated vesicle. Fig 13-14d formation of copii coated vesicle: sec24 and sec23 (coat proteins) are induced by sar1- Gtp to bind to sar1 and cargo receptors bind to adaptor to make an inner coat shown above. Fig 13-16 tethering of vesicle to target membrane. Rab = id marker for organelles and is on both donar and target membranes.