BIOL 3110 Lecture Notes - Lecture 18: Helicase, Green Fluorescent Protein, Mutagen
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Nov 23: muts recognizes a mismatch and binds dna more strongly which makes it stay longer at the error region. When it is bound to adp, it can bind to dna for about 1 sec and cover about 700 bp in a non-specific direction, and then it falls down. Adp for atp and binds to dna stronger to the mutation region, but it still moves around. It will also gain the ability to bind to mutl and mutl can bind muth which is an endonuclease that can cut methylated gatc (a restriction enzyme site that is methylated for bacterial immunity, not methylated in bacteriophages). It takes time for dna to become fully methylated, in the beginning it is hemi-methylated (only the parental strand is methylated). How far does muts before encountering a gatc: a: each base has a probability of , this makes the probability of encountering these four bases.
