BIOL 3110 Lecture 8: Lecture 8 - Molecular Biology I
Document Summary
Real-time using taqman probes: use additional sequence-speci c probe that hybridizes to the pcr products that serve as the template for next round of pcr ampli cation/dna synthesis. Won"t uoresce until you uncouple reporter and quencher. The more product you have, the more rounds of pcr, the more reporter uorescence you have. Examples include satellite, mini-satellite, and micro-satellite dna. Separation based on buoyancy or density (lighter bands that. Oated on top were called satellites; found near centromeres and were found to be repeat sequences) In fact, greater than 40% of the human genome is made up on transposable elements. Transposable elements (te) are also highly repeated sequences found in genomes of complex organisms. In fact, greater than 40@ of the human genome is made up on transposable element. Not all tes are transcribed - regulated by chromatin and epigenetic mechanisms. 5" end of gene is the top of.
