BIOL 3110 Lecture 11: Lecture 11 - Molecular Biology I
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70% based on last bit of material. Includes plasmids in un-replicated form, halfway replicated and fully replicated form: this is so you can visualize how it gets from beginning of s to the end of s phase . Origins of replication in s. cerevisiae have similar architectures, and all contain the. Yeast ars1 = prototypical origin of replication in yeast. A1 + b1 = orc binding site -> note that these two elements alone are not suf cient to initiate dna replication. B3 = abf1 binding site; abf1 = transcription factor that binds to some of the origins in yeast - has auxiliary function. B2 proposed to be where mcm proteins bind. !2: mcm would bind to either side of orc anyway because you need two to start spinning. Mammalian cells tend to initiate dna replication within zones. These replication zones can change in different cell types, development stages, or as a result of mutations:
