BIOL 3155 Lecture Notes - Lecture 6: Reading Frame, Prokaryotic Small Ribosomal Subunit, Cistron
Document Summary
Controlled by rna secondary structure: genome itself is involved in control of translation. Regulate access to ribosome binding sites (rbs: regulating access of host ribosomal machinery to specific sites. Rbs are linear and single stranded - if base paired, they won"t be accessible. Aug start codon in mrna fmet-trna anticodon in the ribosome. Shine-dalgarno (sd) sequences 3" end of 16s rrna. Upstream pyrimidine-rich sequence (up) s1 r-protein (interaction with. Although called upsteam pyrimidine rich sequence, not all nt are pyrimidines rich does not mean exclusively pyrmimidines: binding site(s) need to be ssrna to be bound. Initiator trna is in p site which is complementary to the start codon so it can base pair (rna rna interaction) the 3" end of the 16s component of the small ribosomal subunit is complementary to the. Sd sequence so you get base pairing (rna rna interaction) the s1 ribosomal protein will bind to up (rna protein interaction)