BIOL 4010 Lecture Notes - Lecture 5: Integrase, Avian Sarcoma Leukosis Virus, Colonoscopy
Document Summary
To prove this and figure out activities of genes: mutate the viral genes and hope that one of the mutations would reduce the protein stability at a certain temperature = temperature sensitive mutants. Isolated of rsv mutant (t1 strain) that can replicate and transform the cells at 37 degrees. 42 degrees it loses the ability to transform cells, but it can still replicate the temperature sensitive mutation is not related to the ability of the virus to replicate, but it is important for transformation. Replication and transformation are different activities and require different genes: at 37 degrees, cells infected with mutant virus are transformed. Reduce temperature cells become transformed again. You can make a short probe to find your gene of interest. They were looking for src: take dna samples from cells (infected with rsv and uninfected= control, they thought that uninfected cells would not contain src and the infected cells would have src.
