BIOL 4020 Lecture Notes - Lecture 5: Cpg Site, Dystrophin, Apolipoprotein
23 Nov 2017
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2:34 pm: exp"t: petrie dishes with c321 cells, b/c they have no rf1 or tag - pser(+) system - phosphorylated serine on these proteins. Insert plasmid with paida1 (kanamycin: another plasmid: sepots (ampicillin) - contains trna synthetase, another petrie dish with c321 cells - wild type aida1 protein with normal ser (no phosphate) Human genome: nuclear genome - 26,000 genes + mitochondrial genome - 37 genes & different genetic code, evolved differently. Selection pressure allows one copy to mutate freely, b/c only the other is needed to conserve gene function: the freely mutating copy produces pseudogene - found commonly in eukaryotes. Interspersed repetitive noncoding dna derived mostly from transposons: dna fragments that duplicate themselves and move around our genome, 4 classes of transposons, lines- long interspersed nuclear elements - can make all products necessary for retrotransposition including reverse transcriptase. >6kb long, encode rna binding protein + endonuclease/reverse transcriptase.
