BIOL 4220 Lecture Notes - Lecture 2: Tolonium Chloride, Mast Cell, Aldehyde
Document Summary
Shiff reagent: another histochemical process, its very helpful, but it requires a pre treatment of tissue with something that will render them visible, shiff reagent will bind to aldehyde groups and produce magenta red colors however not many tissue have free aldehyde so what we need to do is to treat the tissue in a way to produce lots of aldehyde groups, usually when you have carbohydrates you can gently oxidize the carbohydrates into aldehydes and the reagent to do this is perodic acid, so treating the tissues with periodic acid first will gently oxidize the carbohydrates into aldehydes but its not strong enough to convert them into acids, we then add the shiff reagent and it will bind to all the aldehyde and this will then stain for basement membranes, and reticular fibers, pas periodic acid shiff, picture (see below lecture notes, ex.
