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Lecture 14

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Department
Natural Science
Course
NATS 1670
Professor
Motti Anafi
Semester
Fall

Description
Transcription Types of RNA mRNA--(messenger) intermediate molecules used for transfer the information from DNA to protein. rRNA--(ribosomal) functional RNA molecules that are components of the ribosome. tRNA--(transfer) functional RNA molecules that serve as adapters in translation. Gene • A region of a DNA molecule containing a sequence of bases that is transcribed into a functional product. • Several regions are responsible for the proper function of a gene. o Regulatory region (promoter)-sequence of bases that control the initiation of transcription. o Transcribed region – sequence of bases that are read into a functional molecule (RNA and protein). o Termination site- sequence of nucleotides that stops transcription. Transcription http://bcs.whfreeman.com/thelifewire/content/chp12/1202001.html • Transcription (copying) is the first step in converting genetic information into proteins • RNA polymerase synthesizes an RNA transcript using one strand of DNA as the template o This strand is the template strand, the other the non-template strand • RNA polymerase synthesizes the RNA strand in the 5’ -> 3’ direction. o This RNA is complementary to the DNA template strand. • Transcription begins nears promoter sites and ends at terminator sites • Three steps of Transcription o Initiation o Elongation o Termination Initiation: How Does Transcription Begin? The enzyme RNA polymerase binds to a specific site in DNA (promoter) with the help of transcription factors http://highered.mcgraw- hill.com/sites/9834092339/student_view0/chapter16/transcription_complex_and_enhancers.htm l Elongation and Termination http://bcs.whfreeman.com/lodish5e/pages/bcs-main_body.asp? s=04000&n=00010&i=04010.01&v=chapter&o=||&ns=0&t=&uid=0&rau=0 • After the enzyme RNA polymerase binds to the promoter, it catalyzes the pr
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