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Lecture 13

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York University
Natural Science
NATS 1670
Motti Anafi

DNAAmplification Polymerase Chain Reaction (PCR) PCR Reaction PCR reaction usually consists of 25 repeating cycles. Each cycle consist of the following stages: • 95 C: the heat denatures the DNA, breaking the bonds that holds the strands together o • 60 C: the primers can anneal o Form hydrogen bonds with their complementary sequences in the target DNA o • 72 C: Taq polymerase begins polymerization. o Adding nucleotides to the 3’ end of each primer attached to DNA strand Beneficial Microbes How to replicate http://highered.mcgraw- Two functional roles of DNA • Duplication: the molecule must be replicated and transmitted in each cell division o Each daughter cell contains almost identical copies of the DNA molecules as compared to its other cell. • Information: The molecule contains information expressed in the sequence of the nucleotides. o The sequences are transcribed into RNA to release their infor
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