NATS 1670 Lecture Notes - Lecture 6: Polymerase Chain Reaction, Swf, Restriction Enzyme
25 Mar 2014
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Restriction enzymes are used to remove a gene. A technique for quickly amplifying and thereby detecting a specific dna sequence http://www. chemie. uni-hamburg. de/bc/praktikum_bc/mb_/pcr3. swf http://highered. mcgraw- hill. com/sites/0072556781/student_view0/chapter14/animation_quiz_6. html. Pcr reaction usually consists of 25 repeating cycles. 95oc: the heat denatures the dna, breaking the bonds that holds the strands together. 60oc: the primers can anneal: form hydrogen bonds with their complementary sequences in the target dna. 72oc: taq polymerase begins polymerization: adding nucleotides to the 3" end of each primer attached to dna strand. Genetic engineering is based on a of technologies. Dna sequencing + recombination of dna + dna amplification =genetic engineering of the 20th century. The 21 st century is about genes, dna and genetic engineering: biotechnology, medicine, agriculture. First drug from genetically engineered animals approved http://reason. com/blog/2009/02/09/first-drug-from-genetically-en. Genetically modified cows may one day produce human breast milk http://phys. org/news/2011-04-genetically-cows-human-breast. html. A genetically modified salmon, back, which can grow twice as fast as a non-transgenic. Atlantic salmon sibling of the same age, front.
