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Lecture 6

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Natural Science
NATS 1670
Motti Anafi

Recombination of DNA DNA from any organism (e.g human) Restriction Enzymes are used to remove a gene DNA from any organism (e.g bacteria) DNA Sequencing Genetic Engineering: DNAAmplification Polymerase Chain reaction (PCR) A technique for quickly amplifying and thereby detecting a specific DNA sequence http://highered.mcgraw- PCR Reaction PCR reaction usually consists of 25 repeating cycles. Each cycle consist of the following stages: o • 95 C: the heat denatures the DNA, breaking the bonds that holds the strands together • 60 C: the primers can anneal o Form hydrogen bonds with their complementary sequences in the target DNA • 72 C: Taq polymerase begins polymerization. o Adding nucleotides to the 3’ end of each primer attached to DNA strand G
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