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Lecture 6

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Department
Natural Science
Course
NATS 1670
Professor
Motti Anafi
Semester
Fall

Description
Recombination of DNA https://www.youtube.com/watch?v=x2jUMG2E-ic DNA from any organism (e.g human) Restriction Enzymes are used to remove a gene DNA from any organism (e.g bacteria) https://www.youtube.com/watch?v=8rXizmLjegI DNA Sequencing http://www.pbslearningmedia.org/asset/tdc02_vid_hgp/ Genetic Engineering: DNAAmplification Polymerase Chain reaction (PCR) A technique for quickly amplifying and thereby detecting a specific DNA sequence http://www.chemie.uni-hamburg.de/bc/Praktikum_BC/MB_/pcr3.swf http://highered.mcgraw- hill.com/sites/0072556781/student_view0/chapter14/animation_quiz_6.html PCR Reaction PCR reaction usually consists of 25 repeating cycles. Each cycle consist of the following stages: o • 95 C: the heat denatures the DNA, breaking the bonds that holds the strands together • 60 C: the primers can anneal o Form hydrogen bonds with their complementary sequences in the target DNA • 72 C: Taq polymerase begins polymerization. o Adding nucleotides to the 3’ end of each primer attached to DNA strand G
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