MELS223 Lecture Notes - Shiga-Like Toxin, Lipopolysaccharide, Methicillin

• School: University of Otago
• Department: Medical Laboratory Science
• Course: MELS223
• Professor: Jenny Rhodes

Document Summary

Hospital microbiology lab looking whether same organism is infecting the patients. What type of human/bac" it is subtyping (strain typing) Dna based method = low level of discrimination. Will tell you some info but not everything. More sophisticated dna-based techniques required = whole genome sequencing. Negative reaction = bacteria just fall to bottom of the well: coli o157:h7. Different bacteriophage may infect different subtypes of 1 species of bacteria ( like staph. aureus) If it is the susceptible, those bacteria will lyse. Phage typed back in 1950 strain 80/81. In 2000, identified as the same phage type acquired a virulence factor panton valentine leucocidin (kills white blood cells) If restriction site missing- will have a larger section of dna. Run through gel electrophoresis relatedness of 1 strand to another. Restaurant chain a" e. coli o157:h7 outbreak identified same strains this way. Infectious otherwise also apart from the hamburger meat. Need endonuclease that only cuts at a few points.