BIOL 1020 Lecture Notes - Thymidine Triphosphate, Genomic Library, Base Pair

• School: Auburn University
• Department: Biology
• Course: BIOL 1020
• Professor: Anne- Marie Singh

Document Summary

Biol 1020 chapter 20 lecture notes chapter 20: dna. General outline of genetic engineering: dna cleavage, production of recombinant dna, cloning of the recombinant dna, screening clones. Dna restriction fragment can form basepairs with the vector: dna ligase is then used to join the dna strand backbones. Dna from these cells is then subjected to other treatments to help identify cell lines containing the dna of interest (for example, the probing covered later in these notes) Techniques used to manipulate and study dna before and after cloning include: pcr, dna gel electrophoresis, probing, 2. iii. typically, the pcr works like this: dna melting is done 94 c (just a bit under the boiling temperature of water) for a minute or less, dna annealing is done at a temperature around. Southern blot or dna gel blot (after the inventor) if rna is being probed, this is called a northern blot or.