BIO 2306 Lecture Notes - Lecture 16: X-Ray, Semiconservative Replication, Protein Structure
Document Summary
Dna and replication: the component d of dna, levene. Determination of chemical components: sugar(5-carbon), phosphate groups, and nitrogenous bases, purines. Idea of complexity: the double rings were more complex than single rings. But he found his idea wrong and discovered that the amount of nucleotide was equal: x ray diffraction. Discovered that the molecule was two helix- secondary protein structure: watson and crick. The backbone was made up of sugar and phosphate group: anti-polar orientation, 5"to 3" end the other strand was 3"to 5" end. The inside was the nitrogenous bases connected through hydrogen bonds. The strands were made by covalent bonds in opposite directions: replication of dna, the faithful replication of dna. Cell free dna synthesis using defined primer: dna of known vase composition is put into a solution containing replicating enzyme and simple nucleoside triphosphates, the nitrobases was equal in the progeny, modes of replication. Semi-conservative replication: the new dna molecule one original and one newly synthesizes dna.