BIO 2306 Lecture Notes - Lecture 20: Whole Genome Sequencing, Human Genome Project, Restriction Map
Document Summary
Content, organization, function, and evolution and genetic information within whole genomes. An individual"s entire genome is the focus. Recombination sequences in genetic crosses (linkage analysis via ch. Measured in cm (centimorgans: physical maps. Can be created by restriction mapping, using restriction enzymes. Whole genome sequencing: determine sequence of entire genome, problems: Only small fragments (500-700bp) can be sequences at a time takes a very long time. Must fragment genome into millions of small overlapping fragments. How to order the fragments once sequences. Goals: determine the sequence of the human genome (3. 2 billion bp, identify all the genes in the human genome (~20k to 25k, develop technology, sequence genomes of model organisms, two projects. Single nucleotide polymorphisms (snips: singe-base-pair differences in dna sequences between individuals of a species, arise through mutation, most do not result in a different phenotype, haplotype, set of snps on a chromosome.
