BIOL3040 Lecture Notes - Lecture 25: Origin Recognition Complex, Cdc6, Cdc25
Document Summary
A temperature-sensitive mutant yeast strain stops proliferating when shifted from 25 c to 37 c. (this usually occurs because there is a mutant protein that can function at. Thus, when grown at 25 c, the phenotype is like wildtype, but when grown at. When these cells are analyzed at the two different temperatures, using a machine that sorts cells according to the amount of dna they contain (facs), the graphs to the right are obtained. Cell cycle control every stage must happen at the right time cell cycle control system: regulates the cycle, 3 checkpoints that the cell is constantly assessing. Is the environment favorable to move onto s phase and replicate dna. Point of no return: either divide or die. Proteins that duplicate dna are turned on or off depending on whether conditions are met to pass the checkpoint: phosphorylation often can turn a protein on or off.