BIOL3190 Lecture Notes - Lecture 30: Cell Adhesion Molecule, International Hapmap Project, Amphoterism
Document Summary
You can take a sample of rnas and analyze them using microarray in which you are comparing 2 samples or you could sequence the rna and plot that sequence against the genome to see which is expressed. Proteomics if you have a protein off a gel and you can take the protein and digest it with trypsin that always cleaves after a lysine and arginine and product has predicted mass that you can measure using peaks. Sds page the way one looks at proteins for 30 years. Nucleic acids are negatively charged and go to negative electrodes. Way they are folded will influence how they will behave. Forces proteins to behave with some regulatory nature. How to use antibodies to identify a protein. You can label a cell by having antibody that recognizes cell surface molecule. 1 antibody that recognizes what we want to study (primary antibody) Secondary antibody has a label and recognizes primary antibody.