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Class Notes for Hoffman

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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 21: Ascus, Leucine, Ura3

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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 19: Escherichia Coli, Lac Repressor, Model Organism

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Fluorescent molecules (gfp: will lose their fluorescence if a light is shined on them too much, also takes a moment for you to be able to see the diffe
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 23: Blastula, Viral Disease, Zinc Finger

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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 10: Restriction Enzyme, Dna Mismatch Repair, Exonuclease

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99. 3% of them get a nucleotide: failing to put a nucleotide changes the length of the whole thing, could have used a better oligote for this sequencin
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 17: Phosphodiester Bond, Electrophoretic Mobility Shift Assay, California Institute Of Technology

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Protein-dna interactions: dnasei footprinting- will show region of protein binding site, ggatcc- palindrome; bahi site, test the sequence specificity o
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 4: Selectable Marker, Base Pair, Sticky And Blunt Ends

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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 17: California Institute Of Technology, Dna-Binding Domain, Phosphodiester Bond

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Protein-dna interactions: dnasei footprinting- will show region of protein binding site, ggatcc- palindrome; bahi site, test the sequence specificity o
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 3: Exonuclease, Endonuclease, Electric Field

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Budding yeast- sacchromyces frogs cerevisiae: knowledge of a candidate cloning of the putative open, mutant strain that cannot grow at. Frog clone- the
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 2: Covalent Bond, Dna Gyrase, Dna Polymerase Iii Holoenzyme

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About the course: focused to set you up to do research, methods oriented, errors in experiments and how to overcome them. Definition assay: improves ab
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 22: Adenine, Ura3, Wild Type

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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 15: Myc, Polyadenylation, Reading Frame

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Distinguish between mapping and cloning: cloning gives you physical access to a physical reagent, library screen by hybridization leads to cloning, sou
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BCBIOL 5060HoffmanSpring

BIOL 5060 Lecture Notes - Lecture 18: Tev Protease, Immunofluorescence, Fusion Protein

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How do we distinguish among these 3 models: 1) you can do another two hybrid with z and y alone, and z and x alone. Or do z and x with and without y. W
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