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BIOL 5060 (27)

Class Notes for BIOL 5060 at Boston College

Recombinant DNA Technology

Exam Study Guides for BIOL 5060

Covers all exam topics.

BIOL 5060 Lecture Notes - Lecture 25: General Idea, Chromosome, Sister Chromatids

5/2_RecDNATech_Hoffman 5/2/2017 4:13:00 PM Scaling Up (is the theme) 1) Synthetic Lethality (something that predates recombinant DNA/ a genetic interaction between mutation...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 24: Omics, Chromatin, Reverse Transcriptase

4/27_RecDNATech_Hoffman 4/27/2017 3:01:00 PM Starting with a mouse knockout- how to assess the effect of the deletion: • 1) Delete one copy in ES cell ...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 25: Reagent, Small Interfering Rna, Rna Interference

4/20_RecDNATech_Hoffman 4/20/2017 3:01:00 PM Goals of Recombinant methods to study gene function • 1) Identify and isolate genes of interest • 2) manipulate ge...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 22: Adenine, Ura3, Wild Type

4/18_RecombinantDNATech_Hoffman 4/18/2017 3:01:00 PM Permissive genes Plasmid shuffle- goal is to generate and identify conditional alleles of a...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 21: Ascus, Leucine, Ura3

4/11_recombinantDNATechnology_Hoffman 4/11/2017 3:01:00 PM What genes interact with my first gene of interest and how do they work together? … we left off with a temperature sensiti...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 20: Pombase, Model Organism, Leucine

4/6_RecombinantDNATech_Hoffman 4/6/2017 2:58:00 PM Yeast Autonomously-Replicating plasmids • Developed to introduce DNA into cells and ALSO in the reverse fas...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 19: Escherichia Coli, Lac Repressor, Model Organism

4/4_RecombinantDNATechnology_Hoffman 4/4/2017 3:00:00 PM Fluorescent Molecules (GFP) • Will lose their fluorescence if a light is shined on them too much • Also takes a m...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 23: Blastula, Viral Disease, Zinc Finger

4/25_RecDNATech_Hoffman 4/25/2017 3:29:00 PM Information ab the promoter • Must be a promoter in the host cell to drive expression of insert gene • ...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 18: Tev Protease, Immunofluorescence, Fusion Protein

3/30_RecombinantDNATechnology_Hoffman 3/30/2017 3:45:00 PM Protein-Protein Interactions Limitations of Two-Hybrid- done in Budding Yeast • Bait itself cannot strongly activ...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 13: Chitin, Epitope, Protein C

3/28_RecombinantDNATechnology 3/28/2017 7:19:00 PM What protein binds to this sequence? • We have knowledge of the cis element not the trans • One hybrid (budding ye...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 17: California Institute Of Technology, Dna-Binding Domain, Phosphodiester Bond

3/23_RecombinantDNATechnology_Hoffman 3/23/2017 2:00:00 PM Protein-DNA interactions • DNaseI footprinting- will show region of protein binding site o GGATCC- ...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 14: Epitope, Affinity Chromatography, Maltose-Binding Protein

3/28_RecombinantDNATechnology 3/28/2017 7:19:00 PM What protein binds to this sequence? • We have knowledge of the cis element not the trans • One hybrid (budding ye...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 17: Phosphodiester Bond, Electrophoretic Mobility Shift Assay, California Institute Of Technology

3/23_RecombinantDNATechnology_Hoffman 3/23/2017 2:00:00 PM Protein-DNA interactions • DNaseI footprinting- will show region of protein binding site o GGATCC- ...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 16: Methylation, Agarose Gel Electrophoresis, Deoxyribonuclease I

3/21_RecombinantDNATechnology_Hoffman 3/21/2017 2:03:00 PM The surface of a protein has the antigenicity that will raise antibodies • = how proteins are detected by antib...

Biology
BIOL 5060
Hoffman
BIOL 5060 Lecture Notes - Lecture 15: Myc, Polyadenylation, Reading Frame

3/16_RecombinantDNATech_Hoffman 3/16/2017 2:01:00 PM Distinguish between mapping and cloning • Cloning gives you physical access to a physical reagent • Library screen...

Biology
BIOL 5060
Hoffman
Permachart - Marketing Reference Guide: Extensor Carpi Ulnaris Muscle, Flexor Pollicis Longus Muscle, Abductor Digiti Minimi Muscle Of Hand

perm ach artsMT T rigger Points II: Extremities T rigger Points II: Extremities ...

ANAT - Anatomy
ANAT 14
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ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
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Permachart - Marketing Reference Guide: Sacral Plexus, Lumbar Plexus, Sympathetic Ganglion

pe rm a ch ar ts MT Spinal Cord 2nd EDITION Figure ...

ANAT - Anatomy
ANAT 14
All Professors
Multicellular Biology - Reference Guides

This Guide is the progressive companion to the Cellular Biology title. The complexities of human physiology are made much clearer through the bright graphics and intelligently coordinated text boxes and glossaries that are...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Levator Veli Palatini, Orbicularis Oculi Muscle, Scalene Muscles

Muscular System: pe rm ac ha rts MT Head & Torso 2nd EDITION ...

ANAT - Anatomy
ANAT 14
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ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
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Permachart - Marketing Reference Guide: Heart Valve, Inferior Vena Cava, Pulmonary Valve

permacharts TM Cardiovascular Physiology 2nd EDITION HEART STRUCTURE & FUNCTION ...

ANAT - Anatomy
ANAT 14
All Professors
ANAT - Anatomy
ANAT 14
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Permachart - Marketing Reference Guide: Meiosis, Nuclear Membrane, Nuclear Pore

l e a r n • r e f e r e n c e • r e v i e w permacharts TM CCellular Biology BIOLOGY BASICS C...

ANAT - Anatomy
ANAT 14
All Professors
Cellular Respiration - Reference Guides

The specific aspects of cellular respiration are explained in this helpful Guide. The fundamental processes such as the electron transport chain, proton pumps, ATP synthetase, and glycolysis are each described with helpful...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Submandibular Duct, Parotid Duct, Palatine Tonsil

pe rm a ch a rtsMT DDigestive System 2nd EDITION Fi...

ANAT - Anatomy
ANAT 14
All Professors
Permachart - Marketing Reference Guide: Antigen-Presenting Cell, Memory T Cell, Clonal Deletion

permacharts TM IImmunology 2nd EDITION G ENERAL INFORMATION - AN OVERVIEW ...

ANAT - Anatomy
ANAT 14
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Permachart - Marketing Reference Guide: Superior Frontal Sulcus, Inferior Parietal Lobule, Superior Frontal Gyrus

p erm ac hartsMT Brain 2nd EDITION FIGURE 1: GROSS ANATOMY (LATERAL VIEW) ...

ANAT - Anatomy
ANAT 173
All Professors
Permachart - Marketing Reference Guide: Superficial Palmar Arch, Costocervical Trunk, Thyrocervical Trunk

p erm a ch ar tsMT CCirculatory System 2nd EDITION Figure...

Biology
BI 361
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