BIOL 5060 Lecture Notes - Lecture 8: Autoradiograph, Comparative Genomics, Intron
Document Summary
We ordered it it doesn"t have methyl groups on the c wasn"t created by us; we bought it from scientist. Based on : 1) expression of a biologically active product- functional cloning, 2) detection of a physical molecule (either a specific seq. of dna. [hybridization] or a protein [immune detection, other metals- in vitro activity]) Example: coli 37 degrees 42 degrees, wt + , mutant + - Why might the second mutant not survive at higher temperatures: probably because the folding of the proteins are denatured, much less likely that the mutant contains an un-functional gene. Genome sequencing increases out understanding of basic biology: comparative genomics- we can compare genomes of different species. Pcr screening can also be used to isolate dna sequences from uncloned genomic dna and cdna: screening the product applies only to expression libraries where the. Dna is expressed to yield a protein: clone can be identified by binding of antibody.
