BIOL 5060 Lecture Notes - Lecture 20: Pombase, Model Organism, Leucine
Document Summary
Leu2 ^(r37a)- means arginine at amino acid 37 is now adenine. Leu2-7- dominant mutant allele: mutant because of allele number, dominant because of caps. Leu2p- represents the protein (not underlined, over the first letter is capitalized) Leu2-3p- mutant protein: fission yeast (genotype) Ura4+- wild-type genes: we don"t use uppercase in fission yeast nomenclature. Ura4-297- mutant allele: since we don"t use uppercase there"s no way of indicating a dominant vs. recessive allele. Leu2, ura3, his3, trp1: fission yeast (pombe) Leu2 (from s. cerevisiae; used in fission yeast to complement a leu1 mutation) His7 lys2: ura3+ and ura4+ both encode omp decarboxylase (part of uracil biosynthetic pathway) Selectable marker- presence is required for growth in medium lacking uracil. Homologous recombination- (another feature related to recombinant dna. Tech); one of the ways to repair damaged dna: 1 centi-morgan- unit of genetic recombination; represents 1% recombination between two locations during meiosis in humans and mice- 1 centi-morgan is about 1 million base pair/
