MCELLBI 104 Lecture Notes - Lecture 4: Expressed Sequence Tag, Shotgun Sequencing, Genomic Library
11 Oct 2016
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Lecture 4: sequencing and assembling genomes ii: annotation. Telomeres and centromeres are both highly repetitive and would make shotgun sequencing difficult but can figure out where they are located. Lots of repetitive pieces = have larger genome library pieces to skip over repetitive sequence. Genome sequence doesn"t tell you much about the function of the genome. Genome annotation: process of attaching biological information to genome sequences (determining which subset of the genome is transcribed) Average gene has 6. 3 transcripts and 20,000 genes. Vast majority of the genome (80%) participates in at least one biochemical rna or chromatin event in at least one cell type. These are not necessarily a required function. Much more of your genome regulates gene expression than codes for proteins. Converting rna transcripts to dna: harvest mrna (blood, spit, purify mrna, reverse transcribe first cdna strand, reverse transcribe second cdna strand. Cdna can be cloned and sequenced just like genomic dna.
