MCELLBI 110 Lecture Notes - Lecture 9: Apoptosis, Uracil, Depurination
24 Feb 2021
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Document Summary
Organisms with large genomes set aside specialized germ cell line: risk of inherited disease, and population variation in general, reflect only the genome sequences transmitted generationally through the germ line. Any cell in the body can be sequenced to inform your inherited disease risk: cancer progression occurs in somatic cells. The cancer genome is not shared with normal surrounding tissue; only biopsy samples with high cancer cell fraction will inform the sequence of the cancer genome. Spontaneous wear and tear creates damaged dna: water can attack at the electronegative carbon attached to o and n, leading to an abasic site, depurination an abasic site, deamination uracil (in dna) Epigenetic marks can alter dna sequence in eukaryotes, conversion to 5-me-c in cpg islands within the promoter regions of genes is one mechanism for silencing expression: modification, deamination. Base oxidation can lead to mispairing: during replication, preferred tautomer.
