MCELLBI 110 Lecture Notes - Lecture 7: Origin Recognition Complex, Orc6, Cdc6

• School: Berkeley
• Department: Molecular And Cell Biology
• Course: MCELLBI 110
• Professor: Thorner

Document Summary

Action of cdc6, another atp binding protein: cdc6 interacts with mcm nd cdt1 that interacts with orc. Bind dna on the inside, cause conformational changes that stimulates atp hydrolysis that allows release of these factors: orc6 subunits loads second molecule of mcm. Once second molecule is loaded, stimulates atp hydrolysis, orc dissociates: leave head to head hexamers, double mcm hexamer on dsdna, load on single strands. In a haploid, the dna content goes from 1n to 2n by the end of s phase; in a diploid, the. Dna content goes from 2n to 4n by the end of s phase. Complementation used to clone a human homolog of the fission yeast cell cycle control gene cdc2: started with a yeast ts mutant. The rise and fall of cyclins drives the temporal program: apc, ubiquitin ligase, properly times the destruction of cyclin.