NUSCTX 170 Lecture Notes - Lecture 3: 18S Ribosomal Rna, Cellular Respiration, Metabolomics
3 Dec 2020
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Lab 3: in vivo methods in experimental research. Indirect calorimetric analysis: body weight monitoring, blood glucose, hormones, lipids, lipoproteins, calorie restriction feeding, core body temperature measurements (cold exposure, fasting, body composition (echomri, high fat diet feeding, fatty acid turnover, glucose tolerance test, pyruvate tolerance test. Indirect calorimetry: real time measurements of oxygen consumption, co2 release, food consumption, temperature control. Body composition echomri: % lean body mass, % fat, % free water, this method uses magnetic resonance to measure free and bound water content in a living subject. What proteins are being produced: metaproteomics, metabolite based approaches, what are the chemical outcomes of their activity, metabolomics. In vitro methods (cell culture studies: o2 consumption, lipid accumulation, lipid mobilization and release. In vitro methods use live cells in culture. Cells can be from an established cell line, or primary cultures derived from biopsies of metabolic tissues.
