BIOL 311 Lecture Notes - Lecture 18: Picea Engelmannii, Purple Sulfur Bacteria, Rhodamine

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Te s t the che mios motic hypothe s is. The diffe re nce in protons + the diffe re nce in vm > adp t-p > atp. 3. ma ke s ome unus ua l pa rticle s out of the mitochondria. 4. put mitochondria in low os mola rity s olution. Is o os motic s olution. us e a n ultra s ound. End up with ins ide out s ub-mitochondria l pa rticle s. Te s t to s e e if it works ! A ) us e a ph indica tor dye - we would e xpe ct it will be ba s ic (it works ) B) add adp a nd phos pha te > ge t atp (it works ) We ha ve a n ins ide out mitochondria l pa rticle , proton gra die nt is gre a te r ins ide tha n outs ide.

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