BIOL 311 Lecture Notes - Lecture 13: Dynabeads, Cell Culture, Apoptosis
11 Mar 2018
School
Department
Course
Professor
Document Summary
Applications, separating apoptosis (dye: annexin 5) and. Number of t cells: fluorescence that can stain y sperm cells, and not x chromosomes. Sperm selection = baby selection: highly debated, and not approved, guava (small facs machine, uses fluorescence, but does not separate cells. Velocity sedimentations: separates cells based on speed, through centrifugal field (1x gravity) Fill with buffer : isotonic to cells, resists change in ph. Cells will separate based on size: larger ones will sink to bottom of funnel faster. Selective surfaces: rely on the ability of protein a to bind to ligand a, cell must demonstrate a difference. Panning: using cell culture dishes, have specific legand at bottom of dish. Magnetic beads: bead names, dynabeads, beads have iron core, can be removed with a magnet, beads have antibody receptors, cell fractionation. Lyse the cells (break-up: sonication sound, destroys all membranes, detergent, solubilize all membranes, trituration (most preferred way, mechanism disruption. Density gradient centrifution: copy from someone.
