BIOL 311 Lecture Notes - Lecture 14: Two-Dimensional Gel Electrophoresis, Gel Electrophoresis, Autoradiograph
11 Mar 2018
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Use slab gel: high molecular weight gets trapped close to the start, and low molecular weight travel furher. Preferred over native: much higher resolution, polymetric nature of proteins. Gel electrophoresis: has gel and power supply. Proteins are separated by isoelectric points: bands move laterally in gel until a specific point become stationary. 2 separation processes: 1st dimensional ief, 2nd dimensional sds. Can add antibodies to surface: use a laser. Tof can be detected and printed out: example, alzheimer"s and maldi-tof. Alzheimer"s linked to -amyloid problem: normal 40 amino acid protein, alz 42 amino acid protein. Maldi-tof can be used to detect alz"s. Put a anti- -amyloid on spot: detects both 40 and 42 aa protein. Maldi/seldi: look at diagram, expensive ~k. Other techniques: autoradiography, use a radioisotope to track a protein (tags protein, densitometry, instrument that can convert bands on a gel (autoradiogram) to a graph.
