BIOL 311 Lecture 15: Note (15)
Document Summary
Mammalian perspective (can also include plants, bacteria, yeast) Definition: ability to grow cells in an artificial environment. History: cell culture started in 1900 because of the reticular vs. neuronal theory (over brain tissue, first experiment: put grounded up brain tissue into blood serum. Cell cultures to test new products limits of cell culture: artificial environment be aware, cells can change genotype over time, hela cell line. Over time, the androgen receptor was lost: many cells don"t exhibit the proper phenotype, ex: mdck cell line ( k stands for kidney, not all cells can grow in culture, hepatocytes (human) Neonatal (new born) do divide: neurons. Dye after a while: feeder layers - used with hesc, layer can be dead or alive. Feed the stem cells: problem: feeder layer are often made with animal cells. Not good: cell viability cell function, human liver cells lose function after 7 days, but look 100% viable (fine)