BIOL 311 Lecture Notes - Lecture 9: Fluorescent Lamp, Spectrofluorometer, Cell Membrane
11 Mar 2018
School
Department
Course
Professor
Document Summary
Hand-held confocal microscope: created by lucid (in rochester, ny, used for confocal biopsy of suspected tissue, non-invasive, can help identify what type of skin cancer. Fluorescence microscopy and associated fluorescence technique fluorescence dyes used to identify molecule and examine change in cell physiology, gene expression: hundreds of different types. Dye gets excited with particular of light, emit of light is longer. Ex: excites at 485 nm (bluish), emits 530 nm: 2 types can see fluorescent light (redish, confocal microscope, fluorescent microscope, application, vital fluorescence microscopy. Fluorochromes on living tissue: fluorescence recover after photobleaching (frap) Membrane fluidity: intracellular injections, fluorescence immunocyto chemistry, special applications (many) Vital fluorescance microscopy: use of florochromes to analyze changes in cell physiology, most flororchromes are not cell membrane permeable, need trick to view inside of cell. Attach acetoxymethyl easter (am) make florochromes pereable. Dye enters and am gets cleaved by esterases. Dye cannot leave cell: examples of dyes, mitochondria dyes.
